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KMID : 1098420100180060379
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Korean Journal of Medicinal Crop Science
2010 Volume.18 No. 6 p.379 ~ p.388
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Molecular Phylogenetic Position of Adenophora racemosa, an Endemic Species in Korea
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Ji Yun-Ui
Moon Byeong-cheol
Lee A-eong
Chun Jin-Mi
Choo Byung-Kil
Kim Ho-kyoung
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Abstract
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Adenophora racemosa is recently reported as a new Korean endemic plant species. However, the phylogenetic relationship of this genus has been controversial due to the morphological similarity and frequent morphological change of aerial parts. To verify the phylogenetic position of Adenophora racemosa and phylogenetic relationship of genus Adenophora, we analyzed the internal transcribed spacer (ITS) sequence of nuclear ribosomal DNA (nrDNA) and random amplified polymorphic DNA (RAPD) using 21 individual of 6 Adenophora species, A. verticillata, A. divaricata, A. racemosa, A. remotiflora, A. stricata and A. tetraphylla. In comparative analysis of the nrDNA-ITS sequences, we could not found not only any species specific nucleotide sequence but also could not estimated their inter or intra species. In the phylogenic analysis based on the RAPD derived DNA polymorphism, Adenophora species were classified into four groups by clustering analysis of the UPGMA. These results suggest that the DNA fingerprinting based on RAPD is more suitable than nrDNA-ITS sequence for the phylogenetic analysis of Adenophora species
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KEYWORD
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Adenophora racemosa, RAPD, nrDNA-ITS, Genetic Relationship
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